Ontology highlight
ABSTRACT:
SUBMITTER: Bae E
PROVIDER: S-EPMC2474475 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Bae Euiyoung E Bannen Ryan M RM Phillips George N GN
Proceedings of the National Academy of Sciences of the United States of America 20080708 28
Engineering proteins for higher thermal stability is an important and difficult challenge. We describe a bioinformatic method incorporating sequence alignments to redesign proteins to be more stable through optimization of local structural entropy. Using this method, improved configurational entropy (ICE), we were able to design more stable variants of a mesophilic adenylate kinase with only the sequence information of one psychrophilic homologue. The redesigned proteins display considerable inc ...[more]