Ontology highlight
ABSTRACT:
SUBMITTER: Hartner FS
PROVIDER: S-EPMC2475614 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Hartner Franz S FS Ruth Claudia C Langenegger David D Johnson Sabrina N SN Hyka Petr P Lin-Cereghino Geoffrey P GP Lin-Cereghino Joan J Kovar Karin K Cregg James M JM Glieder Anton A
Nucleic acids research 20080606 12
Although frequently used as protein production host, there is only a limited set of promoters available to drive the expression of recombinant proteins in Pichia pastoris. Fine-tuning of gene expression is often needed to maximize product yield and quality. However, for efficient knowledge-based engineering, a better understanding of promoter function is indispensable. Consequently, we created a promoter library by deletion and duplication of putative transcription factor-binding sites within th ...[more]