Ontology highlight
ABSTRACT:
SUBMITTER: Krjutskov K
PROVIDER: S-EPMC2475630 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Krjutskov Kaarel K Andreson Reidar R Mägi Reedik R Nikopensius Tiit T Khrunin Andrey A Mihailov Evelin E Tammekivi Veronika V Sork Helena H Remm Maido M Metspalu Andres A
Nucleic acids research 20080605 12
Detection of DNA sequence variation is critical to biomedical applications, including disease genetic identification, diagnosis and treatment, drug discovery and forensic analysis. Here, we describe an arrayed primer extension-based genotyping method (APEX-2) that allows multiplex (640-plex) DNA amplification and detection of single nucleotide polymorphisms (SNPs) and mutations on microarrays via four-color single-base primer extension. The founding principle of APEX-2 multiplex PCR requires two ...[more]