Ontology highlight
ABSTRACT:
SUBMITTER: Fuhrman LE
PROVIDER: S-EPMC2478710 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Fuhrman Laura E LE Shianna Kevin V KV Aballay Alejandro A
PloS one 20080806 8
We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C. elegans mutants that exhibit an enhanced pathogen accumulation (epa) phenotype can be rapidly identified and isolated using a sorting system that allows automation of the analysis, sorting, and dispensing of C. elegans by measuring fluorescent bacteria inside the animals. Furthermore, we validate the use of Amplifluor as a new single nucleot ...[more]