Ontology highlight
ABSTRACT:
SUBMITTER: Kim H
PROVIDER: S-EPMC2479787 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Kim Hyeyeung H Lubman David M DM
Journal of chromatography. A 20080327 1
Multi-dimensional liquid-based separation is required for fractionation and mapping of complex protein mixtures from cells. A method that has been used as the first dimension in such separations is chromatofocusing (CF), which is based on generating a pH gradient on an anion exchange column. The use of pH in the first dimension is essential where pH is a fundamental property of proteins and can provide information on post-translationally modified forms of a protein. In this work, a micro-chromat ...[more]