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A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription.

ABSTRACT: The transcription factor VP1 regulates maturation and dormancy in plant seeds by activating genes responsive to the stress hormone abscisic acid (ABA). Although activation involves ABA-responsive elements (ABREs), VP1 itself does not specifically bind ABREs. Instead, we have identified and cloned a basic region leucine zipper (bZIP) factor, TRAB1, that interacts with both VP1 and ABREs. Transcription from a chimeric promoter with GAL4-binding sites was ABA-inducible if cells expressed a GAL4 DNA-binding domain::TRAB1 fusion protein. Results indicate that TRAB1 is a true trans-acting factor involved in ABA-regulated transcription and reveal a molecular mechanism for the VP1-dependent, ABA-inducible transcription that controls maturation and dormancy in plant embryos.

SUBMITTER: Hobo T

PROVIDER: S-EPMC24822 | biostudies-literature | 1999 Dec

REPOSITORIES: biostudies-literature

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A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription.

Hobo T T Kowyama Y Y Hattori T T

Proceedings of the National Academy of Sciences of the United States of America 19991201 26

The transcription factor VP1 regulates maturation and dormancy in plant seeds by activating genes responsive to the stress hormone abscisic acid (ABA). Although activation involves ABA-responsive elements (ABREs), VP1 itself does not specifically bind ABREs. Instead, we have identified and cloned a basic region leucine zipper (bZIP) factor, TRAB1, that interacts with both VP1 and ABREs. Transcription from a chimeric promoter with GAL4-binding sites was ABA-inducible if cells expressed a GAL4 DNA ...[more]

PMID: 10611387

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