Role of Cj1211 in natural transformation and transfer of antibiotic resistance determinants in Campylobacter jejuni.

ABSTRACT: Campylobacter jejuni, an important food-borne human pathogen, is increasingly resistant to antimicrobials. Natural transformation is considered to be a main mechanism for mediating the transfer of genetic materials encoding antibiotic resistance determinants in C. jejuni, but direct evidence for this notion is still lacking. In this study, we determined the role of Cj1211 in natural transformation and in the development of antibiotic resistance in C. jejuni. Insertional mutagenesis of Cj1211, a Helicobacter pylori ComH3 homolog, abolished natural transformation in C. jejuni. In vitro coculture of C. jejuni strains carrying either kanamycin or tetracycline resistance markers demonstrated the development of progenies that were resistant to both antibiotics, indicating that the horizontal tra

SUBMITTER: Jeon B 

PROVIDER: S-EPMC2493120 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature