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Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria.


ABSTRACT: A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.

SUBMITTER: Quemeneur M 

PROVIDER: S-EPMC2493162 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria.

Quéméneur Marianne M   Heinrich-Salmeron Audrey A   Muller Daniel D   Lièvremont Didier D   Jauzein Michel M   Bertin Philippe N PN   Garrido Francis F   Joulian Catherine C  

Applied and environmental microbiology 20080523 14


A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteo  ...[more]

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