Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.
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ABSTRACT:
SUBMITTER: Morris JJ
PROVIDER: S-EPMC2493173 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
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Facilitation of robust growth of Prochlorococcus colonies and dilute liquid cultures by "helper" heterotrophic bacteria.
Morris J Jeffrey JJ Kirkegaard Robin R Szul Martin J MJ Johnson Zackary I ZI Zinser Erik R ER
Applied and environmental microbiology 20080523 14
Axenic (pure) cultures of marine unicellular cyanobacteria of the Prochlorococcus genus grow efficiently only if the inoculation concentration is large; colonies form on semisolid medium at low efficiencies. In this work, we describe a novel method for growing Prochlorococcus colonies on semisolid agar that improves the level of recovery to approximately 100%. Prochlorococcus grows robustly at low cell concentrations, in liquid or on solid medium, when cocultured with marine heterotrophic bacter ...[more]
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