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Trypanosoma brucei mitochondrial ribosomes: affinity purification and component identification by mass spectrometry.


ABSTRACT: Although eukaryotic mitochondrial (mt) ribosomes evolved from a putative prokaryotic ancestor their compositions vary considerably among organisms. We determined the protein composition of tandem affinity-purified Trypanosoma brucei mt ribosomes by mass spectrometry and identified 133 proteins of which 77 were associated with the large subunit and 56 were associated with the small subunit. Comparisons with bacterial and mammalian mt ribosomal proteins identified T. brucei mt homologs of L2-4, L7/12, L9, L11, L13-17, L20-24, L27-30, L33, L38, L43, L46, L47, L49, L52, S5, S6, S8, S9, S11, S15-18, S29, and S34, although the degree of conservation varied widely. Sequence characteristics of some of the component proteins indicated apparent functions in rRNA modification and processing, protein assembly, and mitochondrial metabolism implying possible additional roles for these proteins. Nevertheless most of the identified proteins have no homology outside Kinetoplastida implying very low conservation and/or a divergent function in kinetoplastid mitochondria.

SUBMITTER: Zikova A 

PROVIDER: S-EPMC2493383 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Trypanosoma brucei mitochondrial ribosomes: affinity purification and component identification by mass spectrometry.

Zíková Alena A   Panigrahi Aswini K AK   Dalley Rachel A RA   Acestor Nathalie N   Anupama Atashi A   Ogata Yuko Y   Myler Peter J PJ   Stuart Kenneth K  

Molecular & cellular proteomics : MCP 20080324 7


Although eukaryotic mitochondrial (mt) ribosomes evolved from a putative prokaryotic ancestor their compositions vary considerably among organisms. We determined the protein composition of tandem affinity-purified Trypanosoma brucei mt ribosomes by mass spectrometry and identified 133 proteins of which 77 were associated with the large subunit and 56 were associated with the small subunit. Comparisons with bacterial and mammalian mt ribosomal proteins identified T. brucei mt homologs of L2-4, L7  ...[more]

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