Ontology highlight
ABSTRACT:
SUBMITTER: Ensign AA
PROVIDER: S-EPMC2504770 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Ensign Amy A AA Jo Iris I Yildirim Ilyas I Krauss Todd D TD Bren Kara L KL
Proceedings of the National Academy of Sciences of the United States of America 20080731 31
FRET between the zinc porphyrin (ZnP) chromophore in zinc-substituted cytochrome c (Zn-cyt c) and an Alexa Fluor dye attached to specific surface sites was used to characterize Zn-cyt c unfolding. The use of ZnP as a fluorescent acceptor eliminates the need to doubly label the protein with exogenous dyes to perform FRET experiments in which both donor and acceptor fluorescence is monitored. The requirement for attachment of only one dye also minimizes perturbation to the protein. This sensitive ...[more]