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An allosteric model of calmodulin explains differential activation of PP2B and CaMKII.

ABSTRACT: Calmodulin plays a vital role in mediating bidirectional synaptic plasticity by activating either calcium/calmodulin-dependent protein kinase II (CaMKII) or protein phosphatase 2B (PP2B) at different calcium concentrations. We propose an allosteric model for calmodulin activation, in which binding to calcium facilitates the transition between a low-affinity [tense (T)] and a high-affinity [relaxed (R)] state. The four calcium-binding sites are assumed to be nonidentical. The model is consistent with previously reported experimental data for calcium binding to calmodulin. It also accounts for known properties of calmodulin that have been difficult to model so far, including the activity of nonsaturated forms of calmodulin (we predict the existence of open conformations in the absence of calcium), an increase in calcium affinity once calmodulin is bound to a target, and the differential activation of CaMKII and PP2B depending on calcium concentration.

SUBMITTER: Stefan MI

PROVIDER: S-EPMC2504824 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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An allosteric model of calmodulin explains differential activation of PP2B and CaMKII.

Stefan Melanie I MI Edelstein Stuart J SJ Le Novère Nicolas N

Proceedings of the National Academy of Sciences of the United States of America 20080731 31

Calmodulin plays a vital role in mediating bidirectional synaptic plasticity by activating either calcium/calmodulin-dependent protein kinase II (CaMKII) or protein phosphatase 2B (PP2B) at different calcium concentrations. We propose an allosteric model for calmodulin activation, in which binding to calcium facilitates the transition between a low-affinity [tense (T)] and a high-affinity [relaxed (R)] state. The four calcium-binding sites are assumed to be nonidentical. The model is consistent ...[more]

PMID: 18669651

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Project description:AII amacrine cells are essential interneurons of the primary rod pathway and transmit rod-driven signals to ON cone bipolar cells to enable scotopic vision. Gap junctions made of connexin36 (Cx36) mediate electrical coupling among AII cells and between AII cells and ON cone bipolar cells. These gap junctions underlie a remarkable degree of plasticity and are modulated by different signaling cascades. In particular, Ca2+/calmodulin-dependent protein kinase II (CaMKII) has been characterized as an important regulator of Cx36, capable of potentiating electrical coupling in AII cells. However, it is unclear which CaMKII isoform mediates this effect. To obtain a more detailed understanding of the isoform composition of CaMKII at retinal gap junctions, we analyzed the retinal distribution of all four CaMKII isoforms using confocal microscopy. These experiments revealed a differential distribution of CaMKII isoforms: CaMKII-? was strongly expressed in starburst amacrine cells, which are known to lack electrical coupling. CaMKII-? was abundant in OFF bipolar cells, which form electrical synapses in the outer and the inner retina. CaMKII-? was diffusely distributed across the entire retina and could not be assigned to a specific cell type. CaMKII-? labeling was evident in bipolar and AII amacrine cells, which contain the majority of Cx36-immunoreactive puncta in the inner retina. We double-labeled retinas for Cx36 and the four CaMKII isoforms and revealed that the composition of the CaMKII enzyme differs between gap junctions in the outer and the inner retina: in the outer retina, only CaMKII-? colocalized with Cx36-containing gap junctions, whereas in the inner retina, CaMKII-? and -? colocalized with Cx36. This finding suggests that gap junctions in the inner and the outer retina may be regulated differently although they both contain the same connexin. Taken together, our study identifies CaMKII-? and -? as Cx36-specific regulators in the mouse retina with CaMKII-? regulating the primary rod pathway.

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An allosteric model of calmodulin explains differential activation of PP2B and CaMKII.

Publications

An allosteric model of calmodulin explains differential activation of PP2B and CaMKII.

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