Ontology highlight
ABSTRACT:
SUBMITTER: Ferreiro DU
PROVIDER: S-EPMC2504826 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Ferreiro Diego U DU Sánchez Ignacio E IE de Prat Gay Gonzalo G
Proceedings of the National Academy of Sciences of the United States of America 20080729 31
We describe the formation of protein-DNA contacts in the two-state route for DNA sequence recognition by a transcriptional regulator. Surprisingly, direct sequence readout establishes in the transition state and constitutes the bottleneck of complex formation. Although a few nonspecific ionic interactions are formed at this early stage, they mainly play a stabilizing role in the final consolidated complex. The interface is fairly plastic in the transition state, likely because of a high level of ...[more]