Ontology highlight
ABSTRACT:
SUBMITTER: Adelman ZN
PROVIDER: S-EPMC2518454 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Adelman Zach N ZN Anderson Michelle A E MA Morazzani Elaine M EM Myles Kevin M KM
Insect biochemistry and molecular biology 20080422 7
The RNA interference pathway functions as an antiviral defense in invertebrates. In order to generate a phenotypic marker which "senses" the status of the RNAi pathway in Aedes aegypti, transgenic strains were developed to express EGFP and DsRED marker genes in the eye, as well as double-stranded RNA homologous to a portion of the EGFP gene. Transgenic "sensor" mosquitoes exhibited robust eye-specific DsRED expression with little EGFP, indicating RNAi-based silencing. Cloning and high-throughput ...[more]