Controlling the shape of filamentous cells of Escherichia coli.
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ABSTRACT:
SUBMITTER: Takeuchi S
PROVIDER: S-EPMC2519610 | biostudies-literature | 2005 Sep
REPOSITORIES: biostudies-literature
Publications
Controlling the shape of filamentous cells of Escherichia coli.
Takeuchi Shoji S DiLuzio Willow R WR Weibel Douglas B DB Whitesides George M GM
Nano letters 20050901 9
This paper describes a technique for growing filamentous cells of Escherichia coli with defined shapes, including crescents, zigzags, sinusoids, and spirals. The procedure begins with the fabrication of embossed microchambers in agarose. Cells are trapped in the chambers by placing a flat, flexible "ceiling", either a slab of agarose or poly(dimethylsiloxane), against an agarose mold on which a suspension of cells has been added; the use of agarose keeps cells hydrated and allows nutrients to di ...[more]
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