Ontology highlight
ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC2525836 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Wang Yongping Y Wang Yun E YE Cotticelli M Grazia MG Wilson Robert B RB
PloS one 20080909 9
RNA interference (RNAi) is a mechanism for inhibiting gene expression through the action of small, non-coding RNAs. Most existing RNAi libraries target single genes through canonical pathways. Endogenous microRNAs (miRNAs), however, often target multiple genes and can act through non-canonical pathways, including pathways that activate gene expression. To interrogate all possible functions, we designed, synthesized, and validated the first shRNA-encoding library that is completely random at the ...[more]