A high-throughput percentage-of-binding strategy to measure binding energies in DNA-protein interactions: application to genome-scale site discovery.
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ABSTRACT:
SUBMITTER: Wang X
PROVIDER: S-EPMC2528174 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
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A high-throughput percentage-of-binding strategy to measure binding energies in DNA-protein interactions: application to genome-scale site discovery.
Wang Xiaohu X Gao Haichun H Shen Yufeng Y Weinstock George M GM Zhou Jizhong J Palzkill Timothy T
Nucleic acids research 20080724 15
Quantifying the binding energy in DNA-protein interactions is of critical importance to understand transcriptional regulation. Based on a simple computational model, this study describes a high-throughput percentage-of-binding strategy to measure the binding energy in DNA-protein interactions between the Shewanella oneidensis ArcA two-component transcription factor protein and a systematic set of mutants in an ArcA-P (phosphorylated ArcA) binding site. The binding energies corresponding to each ...[more]
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