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Comparison of hydroxylated print additives on antibody microarray performance.

ABSTRACT: Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human IL-1beta, IL-4, and TNFalpha, on these microarraying slides.

SUBMITTER: Wu P

PROVIDER: S-EPMC2528199 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature

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Comparison of hydroxylated print additives on antibody microarray performance.

Wu Peng P Grainger David W DW

Journal of proteome research 20061101 11

Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 degrees C for capturing model analytes, anti-human ...[more]

PMID: 17081047

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