Ontology highlight
ABSTRACT:
SUBMITTER: Izumikawa M
PROVIDER: S-EPMC2531066 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
Izumikawa Miho M Cheng Qian Q Moore Bradley S BS
Journal of the American Chemical Society 20060201 5
Benzoic acid priming of the enterocin and actinorhodin type II polyketide synthase complexes was accomplished in vitro via an unprecedented type II nonribosomal peptide synthetase-like mechanism involving the benzoate:acyl carrier protein (ACP) ligase EncN and the ACP EncC. The transfer of the aryl acid to the ACP is ATP-dependent, yet coenzyme A-independent, as characterized with radiolabeled substrates and protein mass spectrometry. Subsequent transport of the ACP-bound aryl group to the nativ ...[more]