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ABSTRACT:
SUBMITTER: Tarnawski M
PROVIDER: S-EPMC2531263 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Tarnawski Miroslaw M Krzywda Szymon S Szczepaniak Andrzej A Jaskolski Mariusz M
Acta crystallographica. Section F, Structural biology and crystallization communications 20080829 Pt 9
RbcX is a dimeric protein found in cyanobacteria that assists in the assembly of the oligomeric RuBisCO complex. RbcX from the thermophile Thermosynechococcus elongatus (TeRbcX) contains an unusual Cys103 residue in its sequence and when expressed recombinantly the protein aggregates and cannot be crystallized. Site-directed mutagenesis of Cys103 to either Arg or Ala produced non-aggregating proteins that could be readily crystallized in several crystal forms. Synchrotron-radiation X-ray diffrac ...[more]