Ontology highlight
ABSTRACT:
SUBMITTER: Sheng J
PROVIDER: S-EPMC2532523 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Sheng Jia J Jiang Jiansheng J Salon Jozef J Huang Zhen Z
Organic letters 20070131 5
[reaction: see text] To investigate nucleic acids with selenium derivatization for crystallography, we report the first synthesis of 2'-methylseleno-thymidine phosphoramidite and its incorporation into DNAs and RNAs by solid-phase synthesis with over 99% coupling yield. The d(GT(Se)GTACAC)2 crystal structure was also determined at 1.40 A resolution using Se phasing, revealing that this Se derivatization did not cause significant structure perturbation, consistent with our UV melting study. In ad ...[more]