Ontology highlight
ABSTRACT:
SUBMITTER: Lewis IA
PROVIDER: S-EPMC2533272 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Lewis Ian A IA Schommer Seth C SC Hodis Brendan B Robb Kate A KA Tonelli Marco M Westler William M WM Sussman Michael R MR Markley John L JL
Analytical chemistry 20071107 24
One-dimensional (1D) (1)H nuclear magnetic resonance (NMR) spectroscopy is used extensively for high-throughput analysis of metabolites in biological fluids and tissue extracts. Typically, such spectra are treated as multivariate statistical objects rather than as collections of quantifiable metabolites. We report here a two-dimensional (2D) (1)H-(13)C NMR strategy (fast metabolite quantification, FMQ, by NMR) for identifying and quantifying the approximately 40 most abundant metabolites in biol ...[more]