Ontology highlight
ABSTRACT:
SUBMITTER: Lin X
PROVIDER: S-EPMC2533732 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Lin Xin X Hopson Russell R Cane David E DE
Journal of the American Chemical Society 20060501 18
The terpene synthase encoded by the SCO5222 (SC7E4.19) gene of Streptomyces coelicolor was cloned by PCR and expressed in Escherichia coli as an N-terminal-His6-tag protein. Incubation of the recombinant protein, SCO5222p, with farnesyl diphosphate (1, FPP) in the presence of Mg(II) gave a new sesquiterpene, (+)-epi-isozizaene (2), whose structure and stereochemistry were determined by a combination of 1H, 13C, COSY, HMQC, HMBC, and NOESY NMR. The steady-state kinetic parameters were kcat 0.049 ...[more]