Ontology highlight
ABSTRACT:
SUBMITTER: Maeder ML
PROVIDER: S-EPMC2535758 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Molecular cell 20080701 2
Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans. A bottleneck in the application of ZFN technology has been the generation of highly specific engineered zinc-finger arrays. Here we describe OPEN (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which we show is more effective than the previously published modular assemb ...[more]