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Luminescent silica nanobeads: characterization and evaluation as efficient cytoplasmatic transporters for T-lymphocytes.

ABSTRACT: We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could be an excellent and nontoxic building block for efficient intracellular transporters.

SUBMITTER: Bottini M

PROVIDER: S-EPMC2536570 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

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Luminescent silica nanobeads: characterization and evaluation as efficient cytoplasmatic transporters for T-lymphocytes.

Bottini Massimo M Cerignoli Fabio F Mills David M DM D'Annibale Federica F Leone Marilisa M Rosato Nicola N Magrini Andrea A Pellecchia Maurizio M Bergamaschi Antonio A Mustelin Tomas T

Journal of the American Chemical Society 20070602 25

We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could b ...[more]

PMID: 17542582

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