Magnetic susceptibility-induced alignment of proteins in reverse micelles.
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ABSTRACT:
SUBMITTER: Valentine KG
PROVIDER: S-EPMC2538951 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Publications
Magnetic susceptibility-induced alignment of proteins in reverse micelles.
Valentine Kathleen G KG Pometun Maxim S MS Kielec Joseph M JM Baigelman Robert E RE Staub Jayme K JK Owens Kristy L KL Wand A Joshua AJ
Journal of the American Chemical Society 20061201 50
Proteins encapsulated within the aqueous core of reverse micelles are found to partially align in a magnetic field. The degree of alignment is sufficient to result in sizable residual 15N-1H dipolar couplings that can be easily measured. It is found that the magnetic susceptibility of the reverse micelle particle is not dominated by the encapsulated protein. The residual dipolar couplings are found to be structurally meaningful. ...[more]
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