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Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR.


ABSTRACT: Three PCR targets (18S ribosomal DNA, B1, and AF146527) and mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different in terms of sensitivity and specificity. However, further analysis highlighted problems sometimes encountered with PCR diagnosis of congenital toxoplasmosis.

SUBMITTER: Filisetti D 

PROVIDER: S-EPMC254317 | biostudies-literature | 2003 Oct

REPOSITORIES: biostudies-literature

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Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR.

Filisetti Denis D   Gorcii Mohamed M   Pernot-Marino Elodie E   Villard Odile O   Candolfi Ermanno E  

Journal of clinical microbiology 20031001 10


Three PCR targets (18S ribosomal DNA, B1, and AF146527) and mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different in terms of sensitivity and specificity. However, further analysis highlighted problems sometimes encountered with PCR diagnosis of congenital toxoplasmosis. ...[more]

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