Ontology highlight
ABSTRACT:
SUBMITTER: Bardet PL
PROVIDER: S-EPMC2544551 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20080908 37
There is a growing interest in the mechanisms that control the apoptosis cascade during development and adult life. To investigate the regulatory events that trigger apoptosis in whole tissues, we have devised a genetically encoded caspase sensor that can be detected in live and fixed tissue by standard confocal microscopy. The sensor comprises two fluorophores, mRFP, monomeric red fluorescent protein (mRFP) and enhanced green fluorescent protein (eGFP), that are linked by an efficient and speci ...[more]