Ontology highlight
ABSTRACT:
SUBMITTER: Yang L
PROVIDER: S-EPMC2544626 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Yang Litao L Ellington Andrew D AD
Analytical biochemistry 20080520 2
We have developed a novel method that uses conformation-switching aptamers for real-time PCR analysis of protein analytes. The aptamers have been designed so that they assume one secondary structure in the absence of a protein analyte and a different secondary structure in the presence of a protein such as thrombin or platelet-derived growth factor (PDGF). The protein-bound structure in turn assembles a ligation junction for the addition of a real-time PCR primer. Protein concentrations could be ...[more]