Ontology highlight
ABSTRACT:
SUBMITTER: Kim HS
PROVIDER: S-EPMC2546753 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Kim Hyong Sun HS Kim Dong-Min DM Neupane Ganesh Prasad GP Lee Yu-mi YM Yang Nam-Woong NW Jang Sook Jin SJ Jung Sook-In SI Park Kyung-Hwa KH Park Hae-Ryoung HR Lee Chang Seop CS Lee Sun Hee SH
Journal of clinical microbiology 20080709 9
We conducted a prospective study to target toxR in the blood of patients with skin and soft tissue infections who were admitted to four tertiary hospitals to assess the clinical usefulness of real-time quantitative PCR (Q-PCR) as a diagnostic technique. We performed conventional PCR (C-PCR), nested PCR (N-PCR), and Q-PCR assays and compared the results to those obtained using the "gold standard" of microbiological culture. The lower detection limit for the Q-PCR assay was 5 x 10(0) copies/microl ...[more]