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ABSTRACT:
SUBMITTER: Rivera M
PROVIDER: S-EPMC2553119 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Rivera Monica M Lee Whasil W Ke Changhong C Marszalek Piotr E PE Cole Daniel G DG Clark Robert L RL
Biophysical journal 20080718 8
In atomic force microscopy-based single molecule force spectroscopy (AFM-SMFS), it is assumed that the pulling angle is negligible and that the force applied to the molecule is equivalent to the force measured by the instrument. Recent studies, however, have indicated that the pulling geometry errors can drastically alter the measured force-extension relationship of molecules. Here we describe a software-based alignment method that repositions the cantilever such that it is located directly abov ...[more]