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Kinetics of charge transfer in DNA containing a mismatch.

ABSTRACT: Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch.

SUBMITTER: Osakada Y

PROVIDER: S-EPMC2553589 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Kinetics of charge transfer in DNA containing a mismatch.

Osakada Yasuko Y Kawai Kiyohiko K Fujitsuka Mamoru M Majima Tetsuro T

Nucleic acids research 20080830 17

Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further ...[more]

PMID: 18757889

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