Ontology highlight
ABSTRACT:
SUBMITTER: Albrecht JW
PROVIDER: S-EPMC2560584 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Albrecht Jacob W JW El-Ali Jamil J Jensen Klavs F KF
Analytical chemistry 20071110 24
This work presents the first implementation of cascaded stages for a microfabricated free-flow isoelectric focusing (FF-IEF) device. Both analytical and computational models for IEF suggest device performance will be improved by utilizing multiple stages to reduce device residence time. These models are shown to be applicable by using focusing of small IEF markers as a demonstration. We also show focusing of fluorescently tagged proteins under different channel geometries, with the most efficien ...[more]