The superiority of polymerase chain reaction over an amplified enzyme immunoassay for the detection of genital chlamydial infections.

ABSTRACT:

Background/objectives

The polymer conjugate enhanced enzyme immunoassay (IDEIA) and Cobas Amplicor polymerase chain reaction Chlamydia trachomatis (CT) (Amplicor PCR) are two commonly used assays for the diagnosis of CT infection. The performance of these assays was compared for the diagnosis of genital CT infection among 1000 consecutive patients attending a genitourinary medicine (GUM) clinic. Confirmation of positive results and the clinical significance of the absence of cryptic plasmid in chlamydia on the diagnosis of infection by Amplicor PCR were also investigated.

Methods

IDEIA, Amplicor PCR, and two nested in-house PCR assays targeting cryptic plasmid and omp1 gene were performed on all samples. DNA from Amplicor PCR negative samples was pooled for in-house PCR assa

SUBMITTER: Jalal H 

PROVIDER: S-EPMC2563809 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature