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A high-throughput assay for siRNA-based circadian screens in human U2OS cells.

ABSTRACT: The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.

SUBMITTER: Vollmers C

PROVIDER: S-EPMC2565498 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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A high-throughput assay for siRNA-based circadian screens in human U2OS cells.

Vollmers Christopher C Panda Satchidananda S DiTacchio Luciano L

PloS one 20081020 10

The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells. ...[more]

PMID: 18941634

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