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Detecting SNPs using a synthetic nanopore.

ABSTRACT: We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the threshold voltage in a synthetic nanopore, it may be possible to discriminate between two variants of the same gene (alleles) that differ in one base.

SUBMITTER: Zhao Q

PROVIDER: S-EPMC2565804 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

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Detecting SNPs using a synthetic nanopore.

Zhao Q Q Sigalov G G Dimitrov V V Dorvel B B Mirsaidov U U Sligar S S Aksimentiev A A Timp G G

Nano letters 20070515 6

We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the t ...[more]

PMID: 17500578

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