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Gene expression profiling in hepatic tissue of newly weaned pigs fed pharmacological zinc and phytase supplemented diets.

ABSTRACT: BACKGROUND: Zinc (Zn) is an essential trace element. However, Zn bioavailability from commonly consumed plants may be reduced due to phytic acid. Zn supplementation has been used to treat diarrheal disease in children, and in the U.S. swine industry at pharmacological levels to promote growth and fecal consistency, but underlying mechanisms explaining these beneficial effects remain unknown. Moreover, adding supplemental phytase improves Zn bioavailability. Thus, we hypothesized that benefits of pharmacological Zn supplementation result from changes in gene expression that could be further affected by supplemental phytase. The goal of this study was to investigate the effects of feeding newly weaned pigs dietary Zn (150, 1,000, or 2,000 mg Zn/kg) as Zn oxide with or without phytase [500 phytase units (FTU)/kg] for 14 d on hepatic gene expression. Liver RNA from pigs fed 150, 1,000, or 2,000 mg Zn/kg, or 1,000 mg Zn/kg with phytase (n = 4 per treatment) was reverse transcribed and examined using the differential display reverse transcription polymerase chain reaction technique. Liver RNA from pigs fed 150 or 2,000 mg Zn/kg (n = 4 per treatment) was also evaluated using a 70-mer oligonucleotide microarray. RESULTS: Expressed sequence tags for 61 putatively differentially expressed transcripts were cloned and sequenced. In addition, interrogation of a 13,297 element oligonucleotide microarray revealed 650 annotated transcripts (FDR

SUBMITTER: Martinez-Montemayor MM

PROVIDER: S-EPMC2566318 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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Gene expression profiling in hepatic tissue of newly weaned pigs fed pharmacological zinc and phytase supplemented diets.

Martínez-Montemayor Michelle M MM Hill Gretchen M GM Raney Nancy E NE Rilington Valencia D VD Tempelman Robert J RJ Link Jane E JE Wilkinson Christopher P CP Ramos Antonio M AM Ernst Catherine W CW

BMC genomics 20080917

<h4>Background</h4>Zinc (Zn) is an essential trace element. However, Zn bioavailability from commonly consumed plants may be reduced due to phytic acid. Zn supplementation has been used to treat diarrheal disease in children, and in the U.S. swine industry at pharmacological levels to promote growth and fecal consistency, but underlying mechanisms explaining these beneficial effects remain unknown. Moreover, adding supplemental phytase improves Zn bioavailability. Thus, we hypothesized that bene ...[more]

PMID: 18799003

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Project description:Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by excretion. An enhanced P availability, and its concomitant supplementation with calcium (Ca), can affect the structure of the microbial community in the digestive tract of broiler chickens. Here, we aim to distinguish the effects of mineral P, Ca, and phytase on the composition of microbial communities present in the content and the mucosa layer of the gastrointestinal tract (GIT) of broiler chickens. Significant differences were observed between digesta and mucosa samples for the GIT sections studied (p = 0.001). The analyses of 56 individual birds showed a high microbial composition variability within the replicates of the same diet. The average similarity within replicates of digesta and mucosa samples across all diets ranged from 29 to 82% in crop, 19-49% in ileum, and 17-39% in caeca. Broilers fed with a diet only supplemented with Ca had the lowest body weight gain and feed conversion values while diets supplemented with P showed the best performance results. An effect of each diet on crop mucosa samples was observed, however, similar results were not obtained from digesta samples. Microbial communities colonizing the ileum mucosa samples were affected by P supplementation. Caeca-derived samples showed the highest microbial diversity when compared to the other GIT sections and the most prominent phylotypes were related to genus Faecalibacterium and Pseudoflavonifractor, known for their influence on gut health and as butyrate producers. Lower microbial diversity in crop digesta was linked to lower growth performance of birds fed with a diet only supplemented with Ca. Each diet affected microbial communities within individual sections, however, no diet showed a comprehensive effect across all GIT sections, which can primarily be attributed to the great variability among replicates. The substantial community differences between digesta and mucosa derived samples indicate that both habitats have to be considered when the influence of diet on the gut microbiota, broiler growth performance, and animal health is investigated.

Project description:Phosphorus pollution caused by animal husbandry is becoming increasingly problematic, especially where decreasing and non-renewable phosphorus resources are concerned. We investigated the growth performance, bone development, phosphorus metabolism and gut microbiota changes elicited by different phosphorus levels with/without phytase in chicks during the brooding period (1-42 d). Five-hundred-and-forty (540) egg-laying chickens were assigned to six groups (0.13% NPP, 0.29% NPP, 0.45% NPP, 0.13% NPP + P, 0.29% NPP + P and 0.45% NPP + P) according to a factorial design with three non-phytate phosphorus (NPP) levels (0.13, 0.29 and 0.45%) and two phytase (P) dosages (0 and 200 FTU/kg). Chicks fed with the diet with 0.13% NPP had the lowest body weight, average daily gain, shank length, average daily feed intake and highest ratio of feed to gain, while phytase supplementation was able to mitigate the adverse effects of low-phosphorus diets on growth performance. Moreover, phosphorus metabolism was affected by different dietary NPP and phytase levels. Thus, 0.13% NPP significantly reduced serum phosphorus, while phytase supplementation significantly increased serum phosphorus. Notably, phosphorus utilization in the 0.13% NPP group was significantly decreased and the phosphorus excretion ratio was increased. Phytase supplementation significantly improved phosphorus utilization by 43.79% and decreased phosphorus emission in the 0.13% NPP group but not in the 0.29% NPP or the 0.45% NPP group. Remarkably, the alpha diversity of gut microbiota was significantly decreased in the low-phosphorus group, while phytase supplementation increased alpha diversity and improved gut microbial community and function. The LEfSe analysis revealed that several differential genera (e.g., Bacteroides, norank_f__Clostridiales_vadinBB60_group and Eggerthella) were enriched in the different dietary NPP and phytase levels. Furthermore, correlations between differential genera and several crucial phenotypes suggested that the enrichment of beneficial bacteria with different levels of phosphorus and phytase promoted phosphorus utilization in the foregut and hindgut. In summary, low-phosphorus diets inhibited growth performance and bone development, decreased utilization of phosphorus and altered gut microbial structure and function in the brooding stage of chicks. Finally, phytase supplementation improves growth performance and bone development and decreases phosphorus emission, and the potential mechanisms may be associated with the reprogramming of gut microbiota.

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Project description:Strategies to reduce economic losses associated with post-weaning diarrhea in pig farming include high-level dietary zinc oxide supplementation. However, excessive usage of zinc oxide in the pig production sector was found to be associated with accumulation of multidrug resistant bacteria in these animals, presenting an environmental burden through contaminated manure. Here we report on zinc tolerance among a random selection of intestinal Escherichia coli comprising of different antibiotic resistance phenotypes and sampling sites isolated during a controlled feeding trial from 16 weaned piglets: In total, 179 isolates from "pigs fed with high zinc concentrations" (high zinc group, [HZG]: n = 99) and a corresponding "control group" ([CG]: n = 80) were investigated with regard to zinc tolerance, antimicrobial- and biocide susceptibilities by determining minimum inhibitory concentrations (MICs). In addition, in silico whole genome screening (WGSc) for antibiotic resistance genes (ARGs) as well as biocide- and heavy metal tolerance genes was performed using an in-house BLAST-based pipeline. Overall, porcine E. coli isolates showed three different ZnCl2 MICs: 128 μg/ml (HZG, 2%; CG, 6%), 256 μg/ml (HZG, 64%; CG, 91%) and 512 μg/ml ZnCl2 (HZG, 34%, CG, 3%), a unimodal distribution most likely reflecting natural differences in zinc tolerance associated with different genetic lineages. However, a selective impact of the zinc-rich supplemented diet seems to be reasonable, since the linear mixed regression model revealed a statistically significant association between "higher" ZnCl2 MICs and isolates representing the HZG as well as "lower ZnCl2 MICs" with isolates of the CG (p = 0.005). None of the zinc chloride MICs was associated with a particular antibiotic-, heavy metal- or biocide- tolerance/resistance phenotype. Isolates expressing the 512 μg/ml MIC were either positive for ARGs conferring resistance to aminoglycosides, tetracycline and sulfamethoxazole-trimethoprim, or harbored no ARGs at all. Moreover, WGSc revealed a ubiquitous presence of zinc homeostasis and - detoxification genes, including zitB, zntA, and pit. In conclusion, we provide evidence that zinc-rich supplementation of pig feed selects for more zinc tolerant E. coli, including isolates harboring ARGs and biocide- and heavy metal tolerance genes - a putative selective advantage considering substances and antibiotics currently used in industrial pork production systems.

Dataset Information

Gene expression profiling in hepatic tissue of newly weaned pigs fed pharmacological zinc and phytase supplemented diets.

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Gene expression profiling in hepatic tissue of newly weaned pigs fed pharmacological zinc and phytase supplemented diets.

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