Ontology highlight
ABSTRACT:
SUBMITTER: Babiarz JE
PROVIDER: S-EPMC2569885 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Babiarz Joshua E JE Ruby J Graham JG Wang Yangming Y Bartel David P DP Blelloch Robert R
Genes & development 20081001 20
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by a complex of TRBP and Dicer. dgcr8Delta/Delta mouse embryonic stem cells (mESCs) have less severe phenotypes than dicer1Delta/Delta mESCs, suggesting a physiological role for Microprocessor-independent, Dicer-dependent small RNAs. To identify these small RNAs with unusual biogenesis, we performed high-throughput sequencing from wild-type, dgcr8Delta/Delta, ...[more]