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Controlled encapsulation of single-cells into monodisperse picolitre drops.

ABSTRACT: Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation.

SUBMITTER: Edd JF

PROVIDER: S-EPMC2570196 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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Controlled encapsulation of single-cells into monodisperse picolitre drops.

Edd Jon F JF Di Carlo Dino D Humphry Katherine J KJ Köster Sarah S Irimia Daniel D Weitz David A DA Toner Mehmet M

Lab on a chip 20080613 8

Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation. ...[more]

PMID: 18651066

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