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Kinetics of Wnt-driven beta-catenin stabilization revealed by quantitative and temporal imaging.

ABSTRACT: The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt-stimulated accumulation of beta-catenin, simultaneously detecting cell number, and it sheds light onto the kinetics of posttranslational stabilization of beta-catenin.

SUBMITTER: Hannoush RN

PROVIDER: S-EPMC2570213 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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Kinetics of Wnt-driven beta-catenin stabilization revealed by quantitative and temporal imaging.

Hannoush Rami N RN

PloS one 20081022 10

The Wnt/beta-catenin signal transduction pathway regulates a broad range of developmental processes. Aberrant activation of the Wnt pathway leads to cancer and degenerative diseases. beta-catenin is a key signaling molecule that is frequently used as a direct monitor of Wnt pathway activation. This paper describes a multi-parametric method for quantitative analysis of cellular beta-catenin protein levels in a rapid and high-throughput manner. The assay offers temporally resolved detection of Wnt ...[more]

PMID: 18941539

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