TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food.
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ABSTRACT:
SUBMITTER: Lambertz ST
PROVIDER: S-EPMC2570298 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
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TaqMan-based real-time PCR method for detection of Yersinia pseudotuberculosis in food.
Lambertz S Thisted ST Nilsson C C Hallanvuo S S
Applied and environmental microbiology 20080829 20
A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail. The complete method, including an internal amplification control, was evaluated for several different food items. ...[more]
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