Ontology highlight
ABSTRACT:
SUBMITTER: Nakajima M
PROVIDER: S-EPMC2570299 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Nakajima Masahiro M Kitaoka Motomitsu M
Applied and environmental microbiology 20080822 20
A beta-1,3-galactosyl-N-acetylhexosamine phosphorylase (GalGlyNAcP) homolog gene was cloned from Vibrio vulnificus CMCP6. In synthetic reactions, the recombinant enzyme acted only with GlcNAc and GalNAc as acceptors in the presence of alpha-d-galactose-1-phosphate as a donor to form lacto-N-biose I (LNB) (Galbeta1 --> 3GlcNAc) and galacto-N-biose (GNB) (Galbeta1 --> 3GalNAc), respectively. GlcNAc was a much better acceptor than GalNAc. The enzyme also phosphorolysed LNB faster than it phosphorol ...[more]