Ontology highlight
ABSTRACT:
SUBMITTER: Vander Kooi CW
PROVIDER: S-EPMC2570371 | biostudies-literature | 2006 Jan
REPOSITORIES: biostudies-literature
Vander Kooi Craig W CW Ohi Melanie D MD Rosenberg Joshua A JA Oldham Michael L ML Newcomer Marcia E ME Gould Kathleen L KL Chazin Walter J WJ
Biochemistry 20060101 1
Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases. Prp19 forms a tetramer via a central coiled-coil domain. Here, we show the U-box domain of Prp19 exists as a dimer within the context of the Prp19 tetramer. A high-resolution structure of the homodimeric state of the Prp19 U-box was determined by X-ray crystallography. Mutation of the U-box dimer interface abrogates U-box dimer formation and is lethal in vivo. The structure of the U-box dimer enables ...[more]