Ontology highlight
ABSTRACT:
SUBMITTER: Bosnakovski D
PROVIDER: S-EPMC2572182 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Bosnakovski Darko D Xu Zhaohui Z Gang Eun Ji EJ Galindo Cristi L CL Liu Mingju M Simsek Tugba T Garner Harold R HR Agha-Mohammadi Siamak S Tassin Alexandra A Coppée Frédérique F Belayew Alexandra A Perlingeiro Rita R RR Kyba Michael M
The EMBO journal 20081002 20
Facioscapulohumeral muscular dystrophy (FSHD) is caused by an unusual deletion with neomorphic activity. This deletion derepresses genes in cis; however which candidate gene causes the FSHD phenotype, and through what mechanism, is unknown. We describe a novel genetic tool, inducible cassette exchange, enabling rapid generation of isogenetically modified cells with conditional and variable transgene expression. We compare the effects of expressing variable levels of each FSHD candidate gene on m ...[more]