Unknown

Dataset Information

0

Structure of the two transmembrane Cu+ transport sites of the Cu+ -ATPases.


ABSTRACT: Cu(+)-ATPases drive metal efflux from the cell cytoplasm. Paramount to this function is the binding of Cu(+) within the transmembrane region and its coupled translocation across the permeability barrier. Here, we describe the two transmembrane Cu(+) transport sites present in Archaeoglobus fulgidus CopA. Both sites can be independently loaded with Cu(+). However, their simultaneous occupation is associated with enzyme turnover. Site I is constituted by two Cys in transmembrane segment (TM) 6 and a Tyr in TM7. An Asn in TM7 and Met and Ser in TM8 form Site II. Single site x-ray spectroscopic analysis indicates a trigonal coordination in both sites. This architecture is distinct from that observed in Cu(+)-trafficking chaperones and classical cuproproteins. The high affinity of these sites for Cu(+) (Site I K(a)=1.3 fM(-1), Site II K(a)=1.1 fM(-1)), in conjunction with reversible direct Cu(+) transfer from chaperones, points to a transport mechanism where backward release of free Cu(+) to the cytoplasm is largely prevented.

SUBMITTER: Gonzalez-Guerrero M 

PROVIDER: S-EPMC2573086 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Structure of the two transmembrane Cu+ transport sites of the Cu+ -ATPases.

González-Guerrero Manuel M   Eren Elif E   Rawat Swati S   Stemmler Timothy L TL   Argüello José M JM  

The Journal of biological chemistry 20080904 44


Cu(+)-ATPases drive metal efflux from the cell cytoplasm. Paramount to this function is the binding of Cu(+) within the transmembrane region and its coupled translocation across the permeability barrier. Here, we describe the two transmembrane Cu(+) transport sites present in Archaeoglobus fulgidus CopA. Both sites can be independently loaded with Cu(+). However, their simultaneous occupation is associated with enzyme turnover. Site I is constituted by two Cys in transmembrane segment (TM) 6 and  ...[more]

Similar Datasets

| S-EPMC2329688 | biostudies-literature
| S-EPMC3537068 | biostudies-literature
| S-EPMC4467857 | biostudies-literature
| S-EPMC5924815 | biostudies-literature
| S-EPMC2742845 | biostudies-literature
| S-EPMC8233418 | biostudies-literature
| S-EPMC5025381 | biostudies-literature
| S-EPMC3092005 | biostudies-literature
| S-EPMC3319165 | biostudies-literature
| S-EPMC4613667 | biostudies-literature