Caenorhabditis elegans mutant allele identification by whole-genome sequencing.
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ABSTRACT:
SUBMITTER: Sarin S
PROVIDER: S-EPMC2574580 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Publications
Caenorhabditis elegans mutant allele identification by whole-genome sequencing.
Sarin Sumeet S Prabhu Snehit S O'Meara M Maggie MM Pe'er Itsik I Hobert Oliver O
Nature methods 20080801 10
Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants. ...[more]
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