Ontology highlight
ABSTRACT:
SUBMITTER: Grueneberg DA
PROVIDER: S-EPMC2575444 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Grueneberg Dorre A DA Degot Sebastien S Pearlberg Joseph J Li Wenliang W Davies Joan E JE Baldwin Amy A Endege Wilson W Doench John J Sawyer Jacqueline J Hu Yanhui Y Boyce Frederick F Xian Jun J Munger Karl K Harlow Ed E
Proceedings of the National Academy of Sciences of the United States of America 20081023 43
shRNA loss-of-function screens were used to identify kinases that were rate-limiting for promoting cell proliferation and survival. Here, we study the differences in kinase requirements among various human cells, including freshly prepared primary cells, isogenic cells, immortalized cells, and cancer cell lines. Closely related patterns of kinase requirements among the various cell types were observed in three cases: (i) in repeat experiments using the same cells, (ii) with multiple populations ...[more]