Unknown

Dataset Information

0

Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.


ABSTRACT: Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N(2) in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression.

SUBMITTER: Olmedo-Verd E 

PROVIDER: S-EPMC2576654 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Role of two NtcA-binding sites in the complex ntcA gene promoter of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.

Olmedo-Verd Elvira E   Valladares Ana A   Flores Enrique E   Herrero Antonia A   Muro-Pastor Alicia M AM  

Journal of bacteriology 20080919 22


Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N(2) in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene d  ...[more]

Similar Datasets

| S-EPMC4248883 | biostudies-literature
| S-EPMC178719 | biostudies-other
| S-EPMC6779454 | biostudies-literature
| S-EPMC5350280 | biostudies-literature
| S-EPMC1544087 | biostudies-literature
| S-EPMC3592012 | biostudies-literature
| S-EPMC4121841 | biostudies-literature
| S-EPMC9457339 | biostudies-literature
| S-EPMC3133150 | biostudies-literature
| S-EPMC7283527 | biostudies-literature