Ontology highlight
ABSTRACT:
SUBMITTER: Iiizumi S
PROVIDER: S-EPMC2577324 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Iiizumi Susumu S Kurosawa Aya A So Sairei S Ishii Yasuyuki Y Chikaraishi Yuichi Y Ishii Ayako A Koyama Hideki H Adachi Noritaka N
Nucleic acids research 20081003 19
In higher animal cells, the principal limitation of gene-targeting technology is the extremely low efficiency of targeted integration, which occurs three to four orders of magnitude less frequently than random integration. Assuming that random integration mechanistically involves non-homologous end-joining (NHEJ), inactivation of this pathway should reduce random integration and may enhance gene targeting. To test this possibility, we examined the frequencies of random and targeted integration i ...[more]