Ontology highlight
ABSTRACT:
SUBMITTER: Jain A
PROVIDER: S-EPMC2581683 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Jain Ankur A Ziegler Jörg J Liscombe David K DK Facchini Peter J PJ Tucker Paul A PA Panjikar Santosh S
Acta crystallographica. Section F, Structural biology and crystallization communications 20081031 Pt 11
A cDNA from the plant Thalictrum flavum encoding pavine N-methyltransferase, an enzyme belonging to a novel class of S-adenosylmethionine-dependent N-methyltransferases specific for benzylisoquinoline alkaloids, has been heterologously expressed in Escherichia coli. The enzyme was purified using affinity and gel-filtration chromatography and was crystallized in space group P2(1). The structure was solved at 2.0 A resolution using a xenon derivative and the single isomorphous replacement with ano ...[more]